Chemical imaging of biological tissue with synchrotron infrared light

Challenges in Biology and Medicine with Synchrotron Infrared Light

The brightness (or brilliance) of synchrotron radiation was exploited in infrared microspectrosocopy. Among application of this synchrotron-based microanalytical technique, biological and biomedical investigations, at the diffraction-limited spot size, are exhibit of an increasing interest among almost all the existing infrared beamline worldwide. This paper is presenting the main properties of...

Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry.

Using synchrotron radiation as an ultra-bright infrared source, we have been able to map the distributions of functional groups such as proteins, lipids, and nucleic acids inside a single living cell with a spatial resolution of a few microns. In particular, we have mapped the changes in the lipid and protein distributions in both the final stages of cell division and also during necrosis.

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom

Analytical Imaging of Cultural Heritage by Synchrotron Radiation and Visible Light - near Infrared Spectroscopy

Imaging is an important tool for analyzing cultural heritage. Due to its delicate nature, the analysis presents numerous technical challenges, probably the most important of which is its requirement for non-destructive and non-invasive investigation. In this study, two techniques used in the analysis of cultural heritage are presented. The first one, synchrotron radiation x-ray fluorescence, is...

Chemical Imaging on Liver Steatosis Using Synchrotron Infrared and ToF-SIMS Microspectroscopies

Fatty liver or steatosis is a frequent histopathological change. It is a precursor for steatohepatitis that may progress to cirrhosis and in some cases to hepatocellular carcinoma. In this study we addressed the in situ composition and distribution of biochemical compounds on tissue sections of steatotic liver using both synchrotron FTIR (Fourier transform infrared) and ToF-SIMS (time of flight...